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A combined RNA preservation and extraction protocol for gene expression studies in cacao beans
Despite the high economic importance of cacao beans, few RNA-based studies have been conducted on this plant material and hence no optimal RNA-extraction has been reported. Moreover, extraction of high-quality RNA from recalcitrant cacao bean tissue…
Selection and validation of reference genes for accurate RT-qPCR gene expression normalization in cacao beans during fermentation
To date, little attention has been paid to the genotypic plasticity and influence of the fermentation process on gene functions and biological processes in cacao beans. The primary tools for such analyses are gene expression studies with reverse…
